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Anti-BTNL2 Antibody (A12078)

Western Blot - Anti-BTNL2 Antibody (A12078) - Antibodies.com Immunohistochemistry - Anti-BTNL2 Antibody (A12078) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to BTNL2 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-BTNL2 Antibody
Description
Rabbit polyclonal antibody to BTNL2.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 246-455 of human BTNL2 (NP_062548.1).
Sequence
LKVNGPSQPILVRVGEDIQLTCYLSPKANAQSMEVRWDRSHRYPAVHVYMDGDHVAGEQMAEYRGRTVLVSDAIDEGRLTLQILSARPSDDGQYRCLFEKDDVYQEASLDLKVVSLGSSPLITVEGQEDGEMQPMCSSDGWFPQPHVPWRDMEGKTIPSSSQALTQGSHGLFHVQTLLRVTNISAVDVTCSISIPFLGEEKIATFSLSGW
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BTL-II, BTNL2_HUMAN, Butyrophilin like 2 (MHC class II associated), Butyrophilin-like protein 2, DAAP-282D22.2, HSBLMHC1, SS2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-BTNL2 Antibody (A12078) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-BTNL2 Antibody (A12078)
Western blot analysis of extracts of various cell lines, using Anti-BTNL2 Antibody (A12078) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-BTNL2 Antibody (A12078) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-BTNL2 Antibody (A12078)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-BTNL2 Antibody (A12078) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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