Anti-Bub3 Antibody [ARC1318] (A307916)

Name: Anti-Bub3 Antibody [ARC1318]
Brand: Antibodies.com
SKU: A307916
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1318] antibody to Bub3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bub3 Antibody [ARC1318]

Description

Rabbit monoclonal [ARC1318] antibody to Bub3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684).

Sequence

MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1318

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Aa2-050, AU019800, AU021329, AU043350, AW146323, BUB 3, BUB 3L, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 mitotic checkpoint protein, BUB3L, BUB3_HUMAN, Budding uninhibited by benomyl, Budding uninhibited by benzimidazoles 3, Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, C78067, hBUB3, Mitotic checkpoint component, Mitotic checkpoint protein bub3, OTTHUMP00000020679, OTTHUMP00000020680, WD repeat type I transmembrane protein A72.5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Bub3 Antibody [ARC1318] (A307916)

Western blot analysis of extracts of various cell lines, using Anti-Bub3 Antibody [ARC1318] (A307916) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Bub3 Antibody [ARC1318] (A307916)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Bub3 Antibody [ARC1318] (A307916) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Bub3 Antibody [ARC1318] (A307916)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Bub3 Antibody [ARC1318] (A307916) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Bub3 Antibody [ARC1318] (A307916)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Bub3 Antibody [ARC1318] (A307916) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.