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Anti-C3a Antibody (A15408)

Western Blot - Anti-C3a Antibody (A6879) - Antibodies.com Immunohistochemistry - Anti-C3a Antibody (A6879) - Antibodies.com Immunohistochemistry - Anti-C3a Antibody (A6879) - Antibodies.com Immunofluorescence - Anti-C3a Antibody (A6879) - Antibodies.com Immunofluorescence - Anti-C3a Antibody (A6879) - Antibodies.com Immunofluorescence - Anti-C3a Antibody (A6879) - Antibodies.com
This product is discontinued

Anti-C3a Antibody (A15408) has been discontinued and is no longer available.

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Name
Anti-C3a Antibody
Description
Rabbit polyclonal antibody to C3a.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:100, IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3 (NP_000055.2).
Sequence
KVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
170kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-C3a Antibody (A6879) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-C3a Antibody (A15408)
Western blot analysis of extracts of various cell lines, using Anti-C3a Antibody (A6879) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry - Anti-C3a Antibody (A6879) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-C3a Antibody (A15408)
Immunohistochemistry of paraffin-embedded rat liver using Anti-C3a Antibody (A6879) at dilution of 1:200 (40x lens).
Immunohistochemistry - Anti-C3a Antibody (A6879) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-C3a Antibody (A15408)
Immunohistochemistry of paraffin-embedded rat kidney using Anti-C3a Antibody (A6879) at dilution of 1:200 (40x lens).
Immunofluorescence - Anti-C3a Antibody (A6879) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-C3a Antibody (A15408)
Immunofluorescence analysis of rat liver using Anti-C3a Antibody (A6879) at dilution of 1:100. Blue: DAPI for nuclear staining.
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