Anti-C3a Antibody [048-18.6.3.X (10-21)] (A278902)

Anti-C3a Antibody [048-18.6.3.X (10-21)]

Mouse monoclonal [048-18.6.3.X (10-21)] antibody to C3a.

This antibody recognises the ~9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer. Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination. Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications.

ELISA, WB

ELISA: 1:1500 - 1:6,000

Human

Native purified human C3a.

Mouse

Monoclonal

048-18.6.3.X (10-21)

IgG1

Protein A affinity chromatography of ascites.

Lot Specific

Liquid

Supplied in Borate Buffered Saline with 0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human C3a antibody, clone 048-18.6.3.X (10-21) recognizes the ~9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination.Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications.