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Anti-C5a-R Antibody (A92299)

Western Blot - Anti-C5a-R Antibody (A92299) - Antibodies.com Western Blot - Anti-C5a-R Antibody (A92299) - Antibodies.com Immunohistochemistry - Anti-C5a-R Antibody (A92299) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to C5a-R for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-C5a-R Antibody
Description
Rabbit polyclonal antibody to C5a-R.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human C5AR1 (NP_001727.2).
Sequence
MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ANAPHYLATOXIN RECEPTOR, C5A, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, C5a ligand, C5aR, c5ar1, C5AR_HUMAN, C5R1, CD88, CD88 antigen, Complement component 5 receptor 1, Complement component 5 receptor 2, Complement component 5a receptor 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-C5a-R Antibody (A92299) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-C5a-R Antibody (A92299)
Western blot analysis of extracts of various cell lines, using Anti-C5a-R Antibody (A92299) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-C5a-R Antibody (A92299) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-C5a-R Antibody (A92299)
Western blot analysis of extracts of various cell lines, using Anti-C5a-R Antibody (A92299) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-C5a-R Antibody (A92299) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-C5a-R Antibody (A92299)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-C5a-R Antibody (A92299) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-C5a-R Antibody (A92299)? Please let us know so that we can list the citation on this page.

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