Anti-CACNA1H Antibody [N55/10] (A304750)

Name: Anti-CACNA1H Antibody [N55/10]
Brand: Antibodies.com
SKU: A304750
Price: 500.0 USD
Availability: InStock

Enlarge Image

$500

Mouse monoclonal [N55/10] antibody to CACNA1H for WB, IHC, ICC/IF, IP and Antibody Microarray.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-CACNA1H Antibody [N55/10]

Description

Mouse monoclonal [N55/10] antibody to CACNA1H.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with Cav1.3.

Immunogen

Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2.

Host

Mouse

Clonality

Monoclonal

Clone ID

N55/10

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~260 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S55-10.

Synonyms

Alpha1 3.2, Alpha13.2, CAC1H_HUMAN, CACNA 1H, CACNA1 H, CACNA1 HB, CACNA1HB, Calcium channel alpha13.2 subunit, Calcium channel voltage dependent T type alpha 1H subunit, Calcium channel, voltage-dependent, T type, alpha 1Hb subunit, Cav 3.2, Cav T.2, Cav3.2, CavT.2, EIG 6, EIG6, Low voltage activated calcium channel alpha 13.2 subunit, Low voltage activated calcium channel alpha1 3.2 subunit, Low-voltage-activated calcium channel alpha1 3.2 subunit, MNCb 1209, T type Cav3.2, Voltage dependent t type calcium channel alpha 1H subunit, Voltage gated calcium channel alpha subunit Cav 3.2, Voltage gated calcium channel alpha subunit Cav T.2, Voltage gated calcium channel alpha subunit Cav3.2, Voltage gated calcium channel alpha subunit CavT.2, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-CACNA1H Antibody [N55/10] (A304750)

Western blot analysis of rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 2 hours at room temperature. Block: 2% Skim Milk + 2% BSA in TBST. The secondary antibody used was Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

Enlarge Image

Figure 2: Immunofluorescence - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNA1H Antibody [N55/10] (A304750), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

Publishing research using Anti-CACNA1H Antibody [N55/10] (A304750)? Please let us know so that we can list the citation on this page.