Anti-CacyBP Antibody (A16264)

Name: Anti-CacyBP Antibody
Brand: Antibodies.com
SKU: A16264
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to CacyBP for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-CacyBP Antibody

Description

Rabbit polyclonal antibody to CacyBP.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CACYBP (NP_055227.1).

Sequence

MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calcyclin binding protein, Calcyclin-binding protein, CYBP_HUMAN, GIG 5, GIG5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein, S100A6-binding protein, S100A6BP, SIAH Interacting Protein, Siah-interacting protein, SIP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-CacyBP Antibody (A16264)

Western blot analysis of extracts of various cell lines, using Anti-CacyBP Antibody (A16264) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CacyBP Antibody (A16264)

Western blot analysis of extracts of various lysates, using Anti-CacyBP Antibody (A16264) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-CacyBP Antibody (A16264)

Western blot analysis of extracts from wild type(WT) and CACYBP knockout (KO) 293T cells, using Anti-CacyBP Antibody (A16264) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 4: Immunofluorescence - Anti-CacyBP Antibody (A16264)

Immunofluorescence analysis of NIH-3T3 cells using Anti-CacyBP Antibody (A16264) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).