CALB1 Rat (A58523)

CALB1 Rat

Calbindin-1 Rat Recombinant

CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.

Escherichia Coli.

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3.

Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

avian-type, CAB27, CALB, CALB 1, CALB1_HUMAN, Calbindin, Calbindin 1 28kDa, Calbindin D28, D 28K, D-28K, D28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL H protein, Vitamin D dependent calcium binding protein, Vitamin D dependent calcium binding protein avian type, Vitamin D-dependent calcium-binding protein