Anti-Caldesmon / CDM Antibody [rCALD1/7266] - BSA and Azide free
Description
Recombinant mouse monoclonal [rCALD1/7266] antibody to Caldesmon / CDM.
Specificity
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Applications
WB, IHC-P
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant human full-length CALD1 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
rCALD1/7266
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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