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Anti-Calponin 1 Antibody [CALP] (A248237)

Immunohistochemistry - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.com Western Blot - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.com Flow Cytometry - Anti-Calponin 1 Antibody [CALP] (A248236) - Antibodies.com Immunofluorescence - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.com SDS-PAGE - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.com
$525
Product Datasheet

Mouse monoclonal [CALP] antibody to Calponin 1 for Flow Cytometry, IF, WB and IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-Calponin 1 Antibody [CALP]
Description
Mouse monoclonal [CALP] antibody to Calponin 1.
Specificity
In western blotting, this antibody only reacts with the 34 kDa form of calponin (and not the 29 kDa form) in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Rat
Immunogen
Crude human uterus extract.
Host
Mouse
Clonality
Monoclonal
Clone ID
CALP
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Calponin 1 Antibody [CALP] - BSA and Azide free (A251420).
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Calponin 1 Antibody [CALP] (A248237)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human uterus using Anti-Calponin 1 Antibody [CALP].
Western Blot - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Calponin 1 Antibody [CALP] (A248237)
Western blot analysis of endometrium lysate using Anti-Calponin 1 Antibody [CALP].
Flow Cytometry - Anti-Calponin 1 Antibody [CALP] (A248236) - Antibodies.comEnlarge Image
Figure 3: Flow Cytometry - Anti-Calponin 1 Antibody [CALP] (A248237)
Flow cytometric analysis of PFA fixed K562 cells using Anti-Calponin 1 Antibody [CALP] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
Immunofluorescence - Anti-Calponin 1 Antibody [CALP] (A248237) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Calponin 1 Antibody [CALP] (A248237)
Immunofluorescent analysis of PFA fixed K562 cells stained with Anti-Calponin 1 Antibody [CALP] followed by Goat Anti-Mouse IgG (CF® 488) (Green). The nuclear counterstain is RedDot.
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