Anti-Calretinin Antibody [ARC53573] (A306902)

Name: Anti-Calretinin Antibody [ARC53573]
Brand: Antibodies.com
SKU: A306902

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This product is discontinued

Anti-Calretinin Antibody [ARC53573] (A306902) has been discontinued and is no longer available.

View all Anti-Calretinin Antibodies.

Specifications

Name

Anti-Calretinin Antibody [ARC53573]

Description

Rabbit monoclonal [ARC53573] antibody to Calretinin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:10,000-1:130,000, IHC: 1:50-1:200, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human Calretinin (NP_001731.2).

Sequence

MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53573

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

29 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

29 kDa calbindin, CAB 29, CAB29, CAL 2, CAL2, CALB 2, CALB2, CALB2_HUMAN, Calbindin 2, Calbindin 2 29kDa, Calbindin D29K, Calbindin2, CR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Calretinin Antibody [ARC53573] (A306902)

Western blot analysis of extracts of various cell lines, using Anti-Calretinin Antibody [ARC53573] (A306902) at 1:110000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Calretinin Antibody [ARC53573] (A306902)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Calretinin Antibody [ARC53573] (A306902) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Calretinin Antibody [ARC53573] (A306902)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Calretinin Antibody [ARC53573] (A306902) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Calretinin Antibody [ARC53573] (A306902)

Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-Calretinin Antibody [ARC53573] (A306902) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.