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Anti-Calsequestrin 1 Antibody [ARC2209] (A305681)

Western Blot - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.com Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.com Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2209] antibody to Calsequestrin 1 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Calsequestrin 1 Antibody [ARC2209]
Description
Rabbit monoclonal [ARC2209] antibody to Calsequestrin 1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (P31415).
Sequence
EDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2209
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Calmitin, Calmitine, Calsequestrin, Calsequestrin 1 (fast twitch, skeletal muscle), Calsequestrin, cell, Calsequestrin, skeletal muscle isoform, Calsequestrin-1, CASQ, Casq1, CASQ1_HUMAN, PDIB1, skeletal muscle isoform
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681)
Western blot analysis of extracts of Rat heart, using Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681)
Immunofluorescence analysis of mouse skeletal muscle using Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Calsequestrin 1 Antibody [ARC2209] (A305681)
Immunofluorescence analysis of rat skeletal muscle using Anti-Calsequestrin 1 Antibody [ARC2209] (A305681) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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