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Anti-CARS Antibody (A91353)

Western Blot - Anti-CARS Antibody (A13676) - Antibodies.com Immunohistochemistry - Anti-CARS Antibody (A13676) - Antibodies.com Immunofluorescence - Anti-CARS Antibody (A13676) - Antibodies.com
This product is discontinued

Anti-CARS Antibody (A91353) has been discontinued and is no longer available.

View all CARS Antibodies.

  • Specifications

Name
Anti-CARS Antibody
Description
Rabbit polyclonal antibody to CARS.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
Sequence
ILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQNGSFQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
85kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CARS1, CysRS, Cysteine--tRNA ligase, cytoplasmic, Cysteinyl-tRNA synthetase
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-CARS Antibody (A13676) - Antibodies.comEnlarge Image

Western Blot - Anti-CARS Antibody (A91353)

Western blot analysis of extracts of various cell lines, using Anti-CARS Antibody (A13676) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Immunohistochemistry - Anti-CARS Antibody (A13676) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-CARS Antibody (A91353)

Immunohistochemistry of paraffin-embedded mouse lung using Anti-CARS Antibody (A13676) at dilution of 1:100 (40x lens).
Immunofluorescence - Anti-CARS Antibody (A13676) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CARS Antibody (A91353)

Immunofluorescence analysis of U2OS cells using Anti-CARS Antibody (A13676). Blue: DAPI for nuclear staining.

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See all CARS Antibodies

Proteins predicted to interact with CARS

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
94.1% Relevancy Score
92.3% Relevancy Score
91.3% Relevancy Score
90.5% Relevancy Score
90.1% Relevancy Score
90% Relevancy Score
89.8% Relevancy Score
89.4% Relevancy Score
89% Relevancy Score