Western Blot - Anti-CASP9 Antibody [Assigned #A10097] (A12789)
Western blot analysis of extracts of various cell lines, using Anti-CASP9 Antibody (A10097) at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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