Anti-Caspase-12 Antibody [ARC58203] (A309297)

Name: Anti-Caspase-12 Antibody [ARC58203]
Brand: Antibodies.com
SKU: A309297

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This product is discontinued

Anti-Caspase-12 Antibody [ARC58203] (A309297) has been discontinued and is no longer available.

View all Anti-Caspase-12 Antibodies.

Specifications

Name

Anti-Caspase-12 Antibody [ARC58203]

Description

Rabbit monoclonal [ARC58203] antibody to Caspase-12.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Caspase-12.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58203

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CASP 12, CASP-12, Casp12, CASP12P1, caspase 12 (gene/pseudogene), caspase 12 pseudogene 1, CASPC_HUMAN, Inactive caspase-12, OTTHUMP00000207032

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Caspase-12 Antibody [ARC58203] (A309297)

Western blot analysis of various lysates, using Anti-Caspase-12 Antibody [ARC58203] (A309297) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Caspase-12 Antibody [ARC58203] (A309297)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Caspase-12 Antibody [ARC58203] (A309297) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Caspase-12 Antibody [ARC58203] (A309297)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Caspase-12 Antibody [ARC58203] (A309297) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Caspase-12 Antibody [ARC58203] (A309297)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Caspase-12 Antibody [ARC58203] (A309297) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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