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Caspase-3 Assay Kit (Colorimetric) (A319626)

Standard Curve - Caspase-3 Assay Kit (Colorimetric) (A319626) - Antibodies.com
$170
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$40 to United States
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7-8 Business Days

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Name
Caspase-3 Assay Kit (Colorimetric)
Description
This Caspase-3 Assay Kit (Colorimetric) is a quantitative assay kit for measuring Caspase-3 activity in animal tissues and cells.
Applications
Functional Studies
Principle of Assay
Caspase 3 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroanilide) by caspase 3, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm.
Sample Type
Animal tissues and cells.
Components
Cell Lysis Buffer, Reaction Buffer (2X), Ac-DEVD-pNA (4mM), pNA (10mM), DTT (100X)
Storage
Shipped at 4°C. Store at -20°C. Do not use past expiration date!
General Notes
96-well microplate is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Caspase-3 Assay Kit (Colorimetric) (A319626) - Antibodies.comEnlarge Image
Figure 1: Standard Curve - Caspase-3 Assay Kit (Colorimetric) (A319626)
Representative pNA standard curve for Caspase-3 Assay Kit (Colorimetric) (A319626).
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