Anti-Caspase-8 Antibody (A80797)

Name: Anti-Caspase-8 Antibody
Brand: Antibodies.com
SKU: A80797
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Caspase-8 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Caspase-8 Antibody

Description

Rabbit polyclonal antibody to Caspase-8.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4-480 of mouse Caspase 8 (O89110).

Sequence

FEQQNHTLEVDSSSHKNYIPDEADFLLGMATVKNCVSYRDPVNGTWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTFTLRKKLFFPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ALPS2B, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, Apoptotic cysteine protease, Apoptotic protease Mch-5, Apoptotic protease Mch5, CAP4, CASP-8, CASP8, CASP8_HUMAN, Caspase 8, Caspase 8 apoptosis related cysteine peptidase, Caspase-8 subunit p10, CED 3, FADD Like ICE, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FLICE, FLJ17672, ICE-like apoptotic protease 5, MACH, MACH alpha 1/2/3 protein, MACH beta 1/2/3/4 protein, MCH5, MGC78473, MORT1 associated ced 3 homolog, MORT1-associated CED-3 homolog, OTTHUMP00000163717, OTTHUMP00000163720, OTTHUMP00000163724, OTTHUMP00000163725, OTTHUMP00000165062, OTTHUMP00000165063, OTTHUMP00000165064, OTTHUMP00000206552, OTTHUMP00000206582

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Caspase-8 Antibody (A80797)

Western blot analysis of extracts of Mouse liver, using Anti-Caspase-8 Antibody (A80797) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Caspase-8 Antibody (A80797)

Western blot analysis of Mouse thymus, using Anti-Caspase-8 Antibody (A80797) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-Caspase-8 Antibody (A80797)

Immunofluorescence analysis of HeLa cells using Anti-Caspase-8 Antibody (A80797) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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