Principle of Assay
Caspase 8 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-IETD-pNA (acetyl-Ile-Glu-Thr-Asp p-nitroanilide) by caspase 8, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm.