This Catalase Activity Assay Kit is a quantitative assay kit for measuring Catalase activity in serum, plasma, animal and plant tissues and cells.
Applications
Functional Studies
Principle of Assay
This kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidase function of catalase for measuring catalase activity.In ther presence of an appropriate concentration of hydrogen peroxide, catalase reacts with methanol to produce formaldehyde which can react with chromogens. The formaldehyde produced can be measured colorimetrically at OD 540 nm.
Sample Type
Serum, plasma, animal tissues, plant tissues, and cells.
Components
Assay Buffer, Sample Diluent, Formaldehyde Standard (4.25 M), Catalase (Control), Potassium Hydroxide, Hydrogen Peroxide, Chromogen, Potassium Periodate
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
96-well microplate / microglass cuvette is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 1: Standard Curve - Catalase Activity Assay Kit (A319666)
Representative formaldehyde standard curve for Catalase Activity Assay Kit (A319666). The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (µM).
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