Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931)

Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin)

Synthetic DNA aptamer to Acute Lymphoblastic Leukemia (CCRF-CEM) [sgc8c] (Biotin).

Recognizes and binds to cell lines Molt-4 (T cell Acute Lymphoblastic Leukemia [ALL]), Sup-T1 (T cell ALL), and Jurkat (T cell ALL).

sgc8c

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Wash Buffer: Dulbecco's Phosphate Buffered Saline with 4.5g/L Glucose, 5mM Magnesium Chloride, Calcium Chloride, and Magnesium Chloride. Binding Buffer: Wash Buffer with 0.1mg/ml Yeast tRNA and 1mg/ml Bovine Serum Albumin. Binding Temperature: 4°C.

5'-dApdTpdCpdTpdApdApdCpdTpdGpdCpdTpdGpdCpdGpdCpdCpdGpdCpdCpdGpdGpdGpdApdApdApdApdTpdApdCpdTpdGpdTpdApdCpdGpdGpdTpdTpdApdGpdAp-3'

41 nt

12,634.26 Da

397600 M^-1cm^-1

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931) is a replica of the aptamer described in PMID: 17373017, 19253922.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.