Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931)

1 Citation
Secondary Structure - Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931) - Antibodies.com
$800
Product Datasheet
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin)
Description
Synthetic DNA aptamer to Acute Lymphoblastic Leukemia (CCRF-CEM) [sgc8c] (Biotin).
Specificity
Recognizes and binds to cell lines Molt-4 (T cell Acute Lymphoblastic Leukemia [ALL]), Sup-T1 (T cell ALL), and Jurkat (T cell ALL).
Aptamer ID
sgc8c
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Wash Buffer: Dulbecco's Phosphate Buffered Saline with 4.5g/L Glucose, 5mM Magnesium Chloride, Calcium Chloride, and Magnesium Chloride. Binding Buffer: Wash Buffer with 0.1mg/ml Yeast tRNA and 1mg/ml Bovine Serum Albumin. Binding Temperature: 4°C.
Sequence
5'-dApdTpdCpdTpdApdApdCpdTpdGpdCpdTpdGpdCpdGpdCpdCpdGpdCpdCpdGpdGpdGpdApdApdApdApdTpdApdCpdTpdGpdTpdApdCpdGpdGpdTpdTpdApdGpdAp-3'
Aptamer Length
41 nt
Aptamer Molecular Weight (Da)
12,634.26 Da
Aptamer Affinity
Affinity (Kd) = 0.78 nM
Extinction Coefficient
397600 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931) is a replica of the aptamer described in PMID: 17373017 and 19253922.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931) - Antibodies.comEnlarge Image

Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931)

Secondary structure of Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931), with a dG of -2.24 kcal/mol.

  • Product Citations (1)

PubMed LogoView Publication
Optimization and modifications of aptamers selected from live cancer cell lines
This publication cites Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931).
Article Abstract

To cut a long story short. Single-stranded DNA aptamers that can recognize cancer cells specifically were optimized and modified. The modifications gave rise to aptamers that were much smaller in size (see scheme) and more stable in fetal bovine serum. This stability makes it possible to apply aptamers to in vivo applications, such as diagnosis and therapy.

View Publication
Publishing research using Anti-Acute Lymphoblastic Leukemia (CCRF-CEM) Aptamer [sgc8c] (Biotin) (A320931)? Please let us know so that we can list the citation on this page.