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Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822)

Secondary Structure - Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin)
Description
Synthetic DNA aptamer to Acute Myeloid Leukemia Cells (HL60) [KH1C12] (Biotin).
Aptamer ID
KH1C12
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: Dulbecco's Phosphate Buffered Saline (containing: Magnesium Chloride, Calcium Chloride), with 4.5g/l Glucose, 5mM Magnesium Chloride, 0.1mg/ml tRNA, 1mg/ml Bovine Serum Albumin, and 20% FBS. Binding Temperature: 4°C.
Sequence
5'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdTpdGpdCpdCpdCpdTpdApdGpdTpdTpdApdCpdTpdApdCpdTpdApdCpdTpdCpdTpdTpdTpdTpdTpdApdGpdCpdApdApdApdCpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp-3'
Aptamer Length
68 nt
Aptamer Molecular Weight (Da)
20,896.62 Da
Aptamer Affinity
Affinity (Kd) = 4.5 nM
Extinction Coefficient
647700 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822) is a replica of the aptamer described in PMID: 19151784.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822) - Antibodies.comEnlarge Image

Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822)

Secondary structure of Anti-Acute Myeloid Leukemia Cells (HL60) Aptamer [KH1C12] (Biotin) (A320822), with a dG of -18.55 kcal/mol.
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