Anti-Chitin Aptamer [Chi#52] (Biotin) (A320944)

Anti-Chitin Aptamer [Chi#52] (Biotin)

Synthetic DNA aptamer to Chitin [Chi#52] (Biotin).

Aptamer also bound to cellulose with 45% efficiency (bound the chitin bead with 73% efficiency). Binding to cellulose was present in each of the selected aptamers and the initial library, suggesting that the primer regions contributed cellulose binding.

Chi#52

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Denatured at 95°C and then cooled to room temperature.

Binding Buffer: 50mM Tris-Acetate, pH 8, with 100mM Sodium Chloride, 100mM Potassium Chloride, and 5mM Magnesium Chloride. Binding Temperature: 25°C.

5'-dTpdApdGpdGpdGpdApdApdTpdTpdCpdGpdTpdCpdGpdApdCpdGpdGpdApdTpdCpdCpdCpdCpdTpdApdApdGpdGpdGpdGpdGpdGpdApdCpdTpdCpdApdGpdCpdApdTpdTpdTpdTpdGpdTpdGpdCpdGpdGpdGpdCpdGpdGpdCpdGpdCpdTpdApdApdCpdApdCpdApdApdTpdCpdApdGpdApdTpdApdGpdApdGpdCpdGpdGpdGpdGpdTpdTpdCpdTpdCpdCpdApdGpdGpdTpdCpdGpdApdCpdGpdCpdApdTpdGpdCpdGpdCpdCpdGp-3'

32,572.08 Da

1004200 M^-1cm^-1

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Anti-Chitin Aptamer [Chi#52] (Biotin) (A320944) is a replica of the aptamer described in PMID: 10743940.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.