Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028)

1 Citation
Secondary Structure - Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) - Antibodies.com
$755
Product Datasheet
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Estrogen Aptamer [BES.1] (Biotin)
Description
Synthetic DNA aptamer to Estrogen [BES.1] (Biotin).
Specificity
Specific to beta-estradiol over deoxycholic acid. Shows cross-reactivity towards dehydroisoandrosterone 3-sulfate and deoxycorticosterone 21-glucoside.
Aptamer ID
BES.1
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C for 5 min and then cooled to room temperature for over 10 min.
Apatmer Binding Conditions
Binding Buffer: 20mM Tris-HCl Buffer, pH 7.4, with 1M Sodium Chloride and 10mM Magnesium Chloride. Binding Temperature: 23°C.
Sequence
5'-dGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdApdTpdGpdGpdApdTpdTpdTpdTpdCpdCpdApdTpdCpdApdApdCpdGpdApdApdGpdTpdGpdCpdGpdTpdCpdCpdGpdTpdCpdCpdCp-3'
Aptamer Length
48 nt
Aptamer Molecular Weight (Da)
14,720.56 Da
Aptamer Affinity
Affinity (Kd) = 6 µM
Extinction Coefficient
446300 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) is a replica of the aptamer described in PMID: 22142383.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) - Antibodies.comEnlarge Image

Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028)

Secondary structure of Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028), with a dG of -12.161 kcal/mol.

  • Product Citations (1)

PubMed LogoView Publication
Optimizing cross-reactivity with evolutionary search for sensors
This publication cites Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028).
Article Abstract

We report a straightforward evolutionary procedure to build an optimal sensor array from a pool of DNA sequences oriented toward three-way junctions. The individual sensors were mined from this pool under separate selection pressures to interact with four steroids, while allowing cross-reactivity, in a manner designed to achieve perfect classification of individual steroids. The resulting sensor array had three sensors and displayed discriminatory capacity between steroid classes over full ranges of concentrations. We propose that similar protocols can be used whenever we have two or more classes of samples, with individual classes being defined through gross differences in ratios of dominant families of responsive components.

View Publication
Publishing research using Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028)? Please let us know so that we can list the citation on this page.