Synthetic DNA aptamer to Estrogen [BES.1] (Biotin).
Specificity
Specific to beta-estradiol over deoxycholic acid. Shows cross-reactivity towards dehydroisoandrosterone 3-sulfate and deoxycorticosterone 21-glucoside.
Aptamer ID
BES.1
Conjugate
Biotin
Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C for 5 min and then cooled to room temperature for over 10 min.
Apatmer Binding Conditions
Binding Buffer: 20mM Tris-HCl Buffer, pH 7.4, with 1M Sodium Chloride and 10mM Magnesium Chloride. Binding Temperature: 23°C.
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) is a replica of the aptamer described in PMID: 22142383.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
![Secondary Structure - Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) - Antibodies.com](https://cdn.antibodies.com/image/catalog/321/A321028_1.jpg?profile=product_top)
![Secondary Structure - Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) - Antibodies.com](https://cdn.antibodies.com/image/catalog/321/A321028_1.jpg?profile=product_top_thumb)
![Secondary Structure - Anti-Estrogen Aptamer [BES.1] (Biotin) (A321028) - Antibodies.com](https://cdn.antibodies.com/image/catalog/321/A321028_1.jpg?profile=product_image)