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Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177)

Secondary Structure - Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin)
Description
Synthetic DNA aptamer to Fibroblast Activation Protein [AptFAP-A4] (Biotin).
Specificity
Claimed to be specific to target over HSA, H-Thr, CD26, Trypsin, CD105.
Aptamer ID
AptFAP-A4
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C for 5 min and then cooled (slowly) to room temperature.
Apatmer Binding Conditions
Phosphate Buffered Saline. Binding Temperature: 23°C.
Sequence
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdCpdCpdGpdCpdApdGpdGpdCpdApdGpdCpdTpdGpdCpdCpdApdTpdTpdApdGpdTpdCpdTpdCpdTpdApdTpdCpdCpdGpdTpdGpdApdCpdGpdGpdTpdApdTpdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
Aptamer Length
80 nt
Aptamer Molecular Weight (Da)
24,681 Da
Aptamer Affinity
Affinity (Kd) = 0.5 nM
Extinction Coefficient
764000 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177) is a replica of the aptamer described in PMID: 36838669.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177) - Antibodies.comEnlarge Image

Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177)

Secondary structure of Anti-Fibroblast Activation Protein Aptamer [AptFAP-A4] (Biotin) (A321177), with a dG of -9.01 kcal/mol.
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