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Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897)

Secondary Structure - Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin)
Description
Synthetic DNA aptamer to Histone H4 N-Terminus [4.33] (Biotin).
Specificity
kD values for other histones (low-/high-salt concentrations): H3 (14/273 nM), H2 A (204/4050 nM), and H2 B (84/825 nM).
Aptamer ID
4.33
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Low-Salt Conditions: 10mM HEPES Buffer, pH 7.5, with 100mM Sodium Chloride and 5mM Magnesium Chloride. High-Salt Conditions: 10mM HEPES Buffer, pH 7.5, with 500mM Sodium Chloride and 10mM Magnesium Chloride.
Sequence
5'-dTpdGpdGpdTpdGpdGpdGpdGpdTpdTpdCpdCpdCpdGpdGpdGpdApdGpdGpdGpdCpdGpdGpdCpdTpdApdCpdGpdGpdGpdTpdTpdCpdCpdGpdTpdApdApdTpdCpdApdGpdApdTpdTpdTpdGpdTpdGpdTp-3'
Aptamer Length
50 nt
Aptamer Molecular Weight (Da)
15,592.13 Da
Aptamer Affinity
Affinity (Kd) = 1.3 nM
Extinction Coefficient
473300 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897) is a replica of the aptamer described in PMID: 22021204.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897) - Antibodies.comEnlarge Image

Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897)

Secondary structure of Anti-Histone H4 N-Terminus Aptamer [4.33] (Biotin) (A320897), with a dG of -5.19 kcal/mol.
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