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Anti-Interferon gamma Aptamer (Biotin) (A321037)

Secondary Structure - Anti-Interferon gamma Aptamer (Biotin) (A321037) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Interferon gamma Aptamer (Biotin)
Description
Synthetic DNA aptamer to Interferon gamma (Biotin).
Specificity
To evaluate the binding specificity of aptamers to target, FITC labeled aptamer was incubated with IFN-c, paraformaldehydefixed IFN-c, trypsin, IgG or BSA coated magnetic beads, respectively. The beads were washed twice with 0.5 ml of binding buffer, suspended in 0.2 ml of binding buffer.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C for 5 min and then cooled (immediately) to 0°C for 15 min.
Apatmer Binding Conditions
Phosphate Buffered Saline with 1mg/ml Bovine Serum Albumin.
Sequence
5'-dCpdCpdGpdCpdCpdCpdApdApdApdTpdCpdCpdCpdTpdApdApdGpdApdGpdApdApdGpdApdCpdTpdGpdTpdApdApdTpdGpdApdCpdApdTpdCpdApdApdApdCpdCpdApdGpdApdCpdApdCpdApdCpdTpdApdCpdApdCpdApdCpdGpdCpdAp-3'
Aptamer Length
59 nt
Aptamer Molecular Weight (Da)
18,001.76 Da
Aptamer Affinity
Affinity (Kd) = 74.5 nM
Extinction Coefficient
585100 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Interferon gamma Aptamer (Biotin) (A321037) is a replica of the aptamer described in PMID: 24849390.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Interferon gamma Aptamer (Biotin) (A321037) - Antibodies.comEnlarge Image

Anti-Interferon gamma Aptamer (Biotin) (A321037)

Secondary structure of Anti-Interferon gamma Aptamer (Biotin) (A321037).
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