Anti-Kanamycin A Aptamer [K16-1] (Biotin) (A321126)

Anti-Kanamycin A Aptamer [K16-1] (Biotin)

Synthetic DNA aptamer to Kanamycin A [K16-1] (Biotin).

K16-1

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

50%: 100?mM Sodium Chloride, pH 7.6, 20?mM Tris Hydrochloride, 2?mM Magnesium Chloride, 5?mM Potassium Chloride, and 1?mM Calcium Chloride. 50%: Cut Smart Buffer, pH 7.9, 50mM Potassium Acetate, 20mM Tris Acetate Buffer, 10mM Magnesium Acetate, and 100 µg/mL Bovine Serum Albumin. Binding Temperature: 20°C.

dCpdGpdCpdApdTpdApdCpdCpdApdGpdCpdTpdTpdApdGpdTpdTpdCpdApdGpdApdApdTpdTpdCpdApdTpdTpdGpdGpdApdGpdCpdGpdTpdGpdGpdCpdGpdTpdGpdGpdApdTpdGpdCpdCpdCpdGpdApdTpdGpdGpdApdCpdCpdGpdCpdCpdCpdCpdApdGpdGpdGpdTpdGpdCpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTpdCpdGpdGpdCp

270 nt

Affinity (Kd) = 342±74 nM

861900 M^-1cm^-1

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.