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Anti-Mycobacterium tuberculosis (H37Rv) Aptamer [NK2] (Biotin) (A320924)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.
Name
Anti-Mycobacterium tuberculosis (H37Rv) Aptamer [NK2] (Biotin)
Description
Synthetic DNA aptamer to Mycobacterium tuberculosis (H37Rv) [NK2] (Biotin).
Specificity
Minimal binding observed against M. bovis Bacillus Guerin (BCG).
Aptamer ID
NK2
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 85°C for 15 min and then snap-cooled on ice for 3 min.
Apatmer Binding Conditions
Binding Buffer: 25mM Tris-HCl, with 50mM Potassium Chloride, 200mM Sodium Chloride, 0.2mM EDTA, 5% (v/v) Glycerol, and 0.5mM DTT. Binding Temperature: 37°C.
Sequence
5'-dGpdCpdGpdGpdGpdApdTpdCpdCpdTpdApdTpdGpdApdCpdGpdCpdApdTpdTpdGpdApdCpdCpdCpdApdCpdApdApdCpdApdCpdApdCpdTpdApdCpdTpdGpdTpdCpdGpdCpdTpdCpdGpdGpdTpdTpdCpdGpdApdApdCpdTpdTpdCpdGpdTpdGpdCpdGpdApdCpdTpdGpdTpdTpdCpdCpdCpdTpdApdTpdApdGpdTpdGpdApdGpdTpdCpdGpdTpdApdTpdTpdApdGpdApdApdTpdTpdCpdCpdGpdCp-3'
Aptamer Length
97 nt
Aptamer Molecular Weight (Da)
29,764.31 Da
Aptamer Affinity
Affinity (Kd) = 31 nM
Extinction Coefficient
909900 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Mycobacterium tuberculosis (H37Rv) Aptamer [NK2] (Biotin) (A320924) is a replica of the aptamer described in PMID: 17442275.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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