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Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867)

Secondary Structure - Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Profenofos Aptamer [SS2-55] (Biotin)
Description
Synthetic DNA aptamer to Profenofos [SS2-55] (Biotin).
Specificity
This aptamer binds preferentially to the organophosphates phorate, profenofos, benzoate and omethoate. It has reduced binding to imidacloprid, acetamiprid, chlorpyrifos, methamidophos, dichlorvos, parathion, dimethoate, monocrotophos and phoxim.
Aptamer ID
SS2-55
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Selection Buffer: 50mM Tris-HCl Buffer, pH 8.3, with 300mM Sodium Chloride, 50mM Potassium Chloride, 10mM Magnesium Chloride. Binding Temperature: 25°C.
Sequence
5'-dApdApdGpdCpdTpdTpdGpdCpdTpdTpdTpdApdTpdApdGpdCpdCpdTpdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdGpdApdTpdCpdGpdGpdApdApdApdApdGpdGpdCpdTpdGpdApdGpdApdGpdCpdTpdApdCpdGpdCp-3'
Aptamer Length
55 nt
Aptamer Molecular Weight (Da)
16,990.09 Da
Aptamer Affinity
Affinity (Kd) = 1 µM
Extinction Coefficient
526900 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867) is a replica of the aptamer described in PMID: 22261866.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867) - Antibodies.comEnlarge Image

Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867)

Secondary structure of Anti-Profenofos Aptamer [SS2-55] (Biotin) (A320867), with a dG of -7.07 kcal/mol.
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