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Anti-ProGRP Aptamer (Biotin) (A320942)

1 Citation
Secondary Structure - Anti-ProGRP Aptamer (Biotin) (A320942) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-ProGRP Aptamer (Biotin)
Description
Synthetic DNA aptamer to ProGRP (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
SELEX Buffer: pH 7.4, with 137mM Sodium Chloride, 8.1mM Disodium Phosphate, 2.68mM Potassium Chloride, 1.47mM Potassium Phosphate, 0.9mM Calcium Chloride, 5.3mM Magnesium Chloride, and 4.5g/l Glucose.
Sequence
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdTpdGpdCpdApdCpdCpdApdCpdTpdTpdApdTpdTpdGpdTpdTpdApdCpdTpdApdApdTpdCpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Aptamer Length
61 nt
Aptamer Molecular Weight (Da)
18,685.27 Da
Aptamer Affinity
Affinity (Kd) = 1.2 µM
Extinction Coefficient
593700 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-ProGRP Aptamer (Biotin) (A320942) is a replica of the aptamer described in PMID: 23179274.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-ProGRP Aptamer (Biotin) (A320942) - Antibodies.comEnlarge Image

Anti-ProGRP Aptamer (Biotin) (A320942)

Secondary structure of Anti-ProGRP Aptamer (Biotin) (A320942), with a dG of -4.96 kcal/mol.

  • Product Citations (1)

PubMed LogoView Publication
Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP), a tumor marker for small cell lung cancer
This publication cites Anti-ProGRP Aptamer (Biotin) (A320942).
Article Abstract

Aptamers are single-strand oligonucleotides that are generated by the systemic evolution of ligands by exponential enrichment (SELEX) technique and that can bind to target molecules specifically. However, only a few aptamers have been developed to date against tumor markers. To utilize aptamers for tumor diagnosis, a variety of aptamers are required. Here, a single-stranded DNA aptamer specific for pro-gastrin-releasing peptide (proGRP), a marker for small cell lung cancer, was selected using SELEX. After selection, identical sequences were found in the DNA library. This sequence was selected and its binding affinity to proGRP was evaluated using surface plasmon resonance.

View Publication
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