Anti-SARS-CoV Helicase Aptamer [NG8] (Biotin) (A321069)

Anti-SARS-CoV Helicase Aptamer [NG8] (Biotin)

Synthetic DNA aptamer to SARS-CoV Helicase [NG8] (Biotin).

NG8

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Denatured at 90°C for 5 min and then cooled at 48°C in Buffer A.

Buffer A (Binding Buffer and Wash Buffer): 50mM Tris-HCl Buffer, pH 6.8, with 150mM Potassium Chloride, 20mM Imidazole, 0.05% Tween 20, and 0.1% Triton X-100. Buffer B (Elution Buffer): 30 ml, 50mM Tris-HCl Buffer, pH 6.8, with 150mM Potassium Chloride, 500mM, Imidazole, 0.02% Tween 20, and 0.1% Triton. Binding Temperature: 25°C.

5'-dCpdCpdGpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdGpdGpdApdGpdCpdTpdCpdGpdGpdTpdApdCpdCpdGpdApdApdTpdTpdCpdApdTpdGpdTpdTpdGpdGpdTpdApdGpdTpdTpdGpdGpdCpdTpdTpdGpdTpdGpdTpdTpdCpdGpdTpdGpdTpdGpdTpdTpdApdApdGpdCpdTpdTpdTpdCpdApdGpdApdGpdApdGpdGpdApdTpdCpdCpdTpdTp-3'

92 nt