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Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819)

Secondary Structure - Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Tenascin C Aptamer [GBI-10] (Biotin)
Description
Synthetic DNA aptamer to Tenascin C [GBI-10] (Biotin).
Specificity
Selected using the TNC positive cell line U251.
Aptamer ID
GBI-10
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 10mM Tris-HCl Buffer, pH 7.2, with 150mM Sodium Chloride, 1mM Magnesium Chloride, 1mM Calcium Chloride, 0.1% (w/v) Bovine Serum Albumin, 0.1 % (v/v) Triton X-100, and 0.1% (v/v) Tween 20. Binding Temperature: 4°C.
Sequence
5'-dGpdGpdCpdTpdGpdTpdTpdGpdTpdGpdApdGpdCpdCpdTpdCpdCpdTpdCpdCpdCpdApdGpdApdGpdGpdGpdApdApdGpdApdCpdTpdTpdTpdApdGpdGpdTpdTpdCpdGpdGpdTpdTpdCpdApdCpdGpdTpdCpdCpdCpdGpdCpdTpdTpdApdTpdTpdCpdTpdTpdApdCpdTpdCpdCpdCp-3'
Aptamer Length
69 nt
Aptamer Molecular Weight (Da)
21,127.69 Da
Aptamer Affinity
Affinity (Kd) = 150 nM
Extinction Coefficient
626300 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819) is a replica of the aptamer described in PMID: 14676325.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819) - Antibodies.comEnlarge Image

Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819)

Secondary structure of Anti-Tenascin C Aptamer [GBI-10] (Biotin) (A320819), with a dG of -16.79 kcal/mol.
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