This 30 Minute IHC DAB Detection System is a powerful intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods and results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.
Applications
IHC
Principle of Assay
The principle of this 30 Minute IHC DAB Detection System is that Linker 1 introduces HRP into the system, Linker 2 introduces Biotin into the system, and Tracer introduces Streptavidin and additional HRP into the system. This 30 Minute IHC DAB Detection System is an enzyme mediated reaction that utilizes horseradish peroxidase (HRP) to catalyze the deposition of two separate Linkers applied sequentially onto tissue sections or cell preparations. The deposited Linkers can be detected with a Streptavidin Peroxidase conjugate followed by a reaction with a peroxidase substrate/chromogen solution such as diaminobenzidine (DAB).
Formulation
All reagent components are formulated without Sodium Azide or Thimerosol as preservatives. The reagents are provided in Ready-to-Use, with the exception of Stable DAB/Plus.
Storage
Store at 2-8°C in the dark.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - 30 Minute IHC DAB Detection System (A320597)
Chromogenic IHC for pan Cytokeratin, detected using either the 30 Minute IHC DAB Detection System (A320597) (right) or a conventional two-step detection system (left).
Immunohistochemistry - 30 Minute IHC DAB Detection System (A320597)
Chromogenic IHC for Ki67, detected using either the 30 Minute IHC DAB Detection System (A320597) (right) or a conventional two-step detection system (left).
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