This 6-Phosphofructokinase Activity Assay Kit is a quantitative assay kit for measuring 6-Phosphofructokinase activity in serum, plasma, animal and plant tissues, cells, cell culture supernatant, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Phosphofructokinase catalyzes formation of fructose-1,6-diphosphate and ADP from fructose-6-phosphate and ATP. Coupled enzymatic reactions consume NADH, and the decrease in absorbance at 340 nm is used to calculate enzyme activity.