ACE-mediated substrate conversion is coupled to reduction of a WST tetrazolium salt to a colored formazan. Inhibition of ACE decreases formazan formation, allowing quantitative determination of ACE inhibitory activity.
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Representation of inhibition measurement. Absorbance (AU) of the sample is compared with Blank 1 (no inhibition), and the inhibited amount is calculated relative to the baseline (Blank 2).