This Acetyl Coenzyme A Assay Kit is a quantitative assay kit for measuring Acetyl-CoA in various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This assay couples acetyl-CoA utilization by citrate synthase with NADH production through malate dehydrogenase. The amount of NADH generated, measured at 340 nm, is proportional to the acetyl-CoA concentration in the sample.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal or plant tissues and cultured cells
Assay Time
40-80 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Standard
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well UV microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Acetyl Coenzyme A Assay Kit (A319654)
Representative standard curve for Acetyl Coenzyme A Assay Kit (A319654). The x-axis is OD340 and the y-axis is acetyl coenzyme A concentration (nmol/mL).
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