This Aconitase Activity Assay Kit is a quantitative assay kit for measuring Aconitase activity in serum, plasma, tissues, cells and plants.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Aconitase activity is measured through conversion of citrate to isocitrate, coupled to NAD+ reduction. The resulting NADH formation is quantified by the increase in absorbance at 340 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, tissues, cells, and plants
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V, Reagent VI
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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