Principle of Assay
This kit provides a simple, convenient and rapid aconitase (ACO) activity detection method, which is suitable for the detection in animal tissues, plant tissues, cells, serum, plasma and other samples. The principle is that ACO catalyzes the conversion of citric acid into isocitrate, and the oxidative decarboxylation of isocitrate reduces NAD+ to NADH, resulting in an increase in light absorption at 340 nm.