This Alcohol Acyltransferase Activity Assay Kit is a quantitative assay kit for measuring Alcohol Acyltransferase activity in serum, plasma, animal and plant tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit provides a convenient tool for detection of alcohol acyltransferase (AAT) activity. The principle is that AAT catalyzes the transfer of the acetyl group from acetyl CoA to butanol, and at the same time reduces DTNB to TNB. TNB has an absorption peak at 412 nm. The enzyme activity of AAT is calculated by measuring the rate of increase in absorption at 412 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and bacteria.
Components
Extraction Buffer, Receptor, Substrate, Chromogen
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!