This alpha Amylase Activity Assay Kit is a quantitative assay kit for measuring alpha Amylase activity in serum, plasma, saliva, animal tissues, and plant tissues (seeds or newly germinated seedings).
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
alpha-Amylase hydrolyzes starch to generate reducing sugars, which react with DNS to form a brown-red product. The increase in absorbance at 540 nm is used to calculate enzyme activity after heat treatment that inactivates beta-amylase.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, saliva, animal tissues, and plant tissues
Assay Time
40-80 minutes
Components
DNS Reagent, Substrate, Standard
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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