Principle of Assay
This kit provides a simple, convenient, and rapid method for determining amino acids, which is suitable for the detection of serum (plasma), animal and plant tissues, cells, cell supernatants, bacteria, and urine. The principle is that the alpha-amine of amino acids can react with ninhydrin hydrate to produce a blue-purple substance, which has a maximum absorption peak at 570 nm. The amino acid content of the sample can be calculated by measuring the absorbance at 570 nm.
