Amino Anchor Intact Universal Protein Cleanup Kit (A334651)

Amino Anchor Intact Universal Protein Cleanup Kit

This kit rapidly captures all proteins from biological samples, free of contaminants for mass spectrometry or downstream proteomics analyses.

Mass Spec

The Amino Anchor Intact Universal Protein Cleanup Kit uses the bifunctional chemistry of the Amino Anchor to prepare pure, high quality protein representing the entire proteome for mass spectrometry or other downstream proteomics analysis. First, proteins are labeled with the Amino Anchor via its amine-reactive group. Via the resin-reactive group of the Amino Anchor, the protein is then captured by the addition of the capture resin, allowing removal of contaminants by washing. Pure protein can is then eluted for downstream proteomics analysis. This streamlined workflow generates intact protein in just a few hours, yields unbiased and unmodified protein samples, and is compatible with most tissues and cell lysates.

Amino Anchor, Lysis buffer, DTT, IAA, Wash buffer 1, Wash buffer 2, MS-compatible elution buffer, Non-MS elution buffer, Capture resin, 2 ml waste collection tubes, 1.5ml low protein-binding tubes, 0.5ml resin capture tubes

Liquid and Resin

Store lysis buffer and elution buffer at room temperature. Store DTT and Amino Anchor at -20°C. All other components should be stored at 4°C.

Protocol optimized for 1-40 µg protein. This kit contains reagents for 8 preparations.