Amino Anchor Peptide Universal Protein Cleanup Kit
Description
This kit rapidly captures and digests all proteins in a sample to peptides, free of contaminants for downstream mass spectrometry.
Applications
Mass Spec
Principle of Assay
The Amino Anchor Intact Universal Protein Cleanup Kit uses the bifunctional chemistry of the Amino Anchor to prepare pure, high quality peptides representing the entire proteome for mass spectrometry analysis. First, proteins are labeled with the Amino Anchor via its amine-reactive group. Via the resin-reactive group of the Amino Anchor, the protein is then captured by the addition of the capture resin, allowing removal of contaminants by washing. Pure protein can be eluted at this point, or it can be digested into peptides with trypsin first and then eluted for MS analysis. This streamlined workflow generates intact protein or MS-ready peptides in just a few hours, yields unbiased and unmodified protein samples, and is compatible with most tissues and cell lysates.
Sample Type
Cell lysate, tissue lysate or other protein source
Amino Anchor Peptide Universal Protein Cleanup Kit (A334652)
Amino Anchor Peptide Universal Protein Cleanup Kit workflow. After labeling proteins in lysate with the Amino Anchor, capture resin binds to protein, enabling removal of contaminants. Intact total protein can then be eluted or trypsin-digested for mass spectrometry analysis.
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