This kit detects apoptotic and necrotic cells using EGFP-labeled Annexin V and Propidium Iodide for fluorescence microscopy or flow cytometry analysis.
Annexin V-EGFP binds phosphatidylserine exposed on apoptotic cells, while propidium iodide stains cells with compromised membranes. This dual-staining approach enables discrimination of viable, early apoptotic, and dead cells by flow cytometry or fluorescence microscopy.
Platform
Flow cytometer, Fluorescence microscope
Sample Type
Live or fixed cultured cells
Assay Time
30-60 minutes
Components
Annexin V Binding Buffer (5X), Annexin V-EGFP, Propidium Iodide (PI), Apoptosis Inducer A, Apoptosis Inducer B
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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