APC is significantly more sensitive than conventional organic fluorophores and has been used in applications such as Flow Cytometry, Homogeneous FRET assay, and Immunoassays. APC's absorbance maximum is at 650 nm, with a resolvable emission peak at 660 nm. It shows minimal fluoresence quenching.
APC won't penetrate into the cell and cause a false-positive reaction.
Non-toxic, with less chance of affecting characteristics of the sample, human body, and environment.
Sample of Protocol
1. Add the Modifier Solution into the antibody.
2. Incubate the reaction at room temperature for 30 min.
3. Add the Pre-activated R-PE and incubate for 1 hour at room temperature, with agitation and protected from light.
4. Add Quencher into the conjugation mixture and mix completely.
5. Incubate at room temperature and keep away from light for 30 min, until the conjugation is complete.
