Highly sensitive WST-8–based colorimetric assay for cell viability and proliferation.
Applications
Functional Assays, Functional Studies, Cell Counting Kit Series
Principle of Assay
Cellular dehydrogenases reduce WST-8 to a soluble orange formazan dye. The amount of formazan produced is directly proportional to the number of metabolically active cells.
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Dose-dependent effects of mitomycin C on HeLa cells measured by Cell Counting Kit-8 (450 nm) and Cytotoxicity LDH Assay Kit-WST (490 nm). Increasing drug concentration decreases cell viability (CCK-8) and increases cytotoxicity (LDH release).
Combined use of Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST with the same samples. Supernatant is used to measure LDH release from dead cells (cytotoxicity), while remaining cells are analyzed for viability using CCK-8. Both assays are based on WST reduction to formazan via NADH-dependent reactions.
Evaluation of cell proliferation and cytotoxicity using CCK-8. Left: IL-2–induced proliferation of CTLL-2 cells showing a dose-dependent increase in absorbance (450 nm). Right: TNF-a–induced cytotoxicity in HeLa cells demonstrating a dose-dependent decrease in cell viability.
