This kit labels the plasma membrane with an orange fluorescent dye for clear observation of cell morphology under fluorescence microscopy.
Applications
Fluorescence Microscopy, Confocal Microscopy, Flow Cytometry, High Content Imaging
Assay Type
Quantitative
Principle of Assay
This kit uses a proprietary orange lipophilic carbocyanine dye that exhibits low fluorescence in water but strong membrane-associated fluorescence after insertion into the plasma membrane. It supports rapid staining of live or fixed cells for fluorescence imaging, flow cytometry, and microplate assays.
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Adherent and suspension cells, live or fixed
Assay Time
15-30 minutes
Components
Orange Dye (1000X), Assay Buffer (10X)
Absorption Maxima
549 nm
Emission Maxima
569 nm
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Hela cells stained using Cell Plasma Membrane Staining Kit (Orange Fluorescence) (A319611).
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