Dual-fluorescence kit for simultaneous detection of live and dead cells.
Applications
Flow Cytometry, FACS, Fluorescence Microscopy, Confocal Microscopy, High Content Imaging, Functional Assays, Cell Based Assay
Principle of Assay
Viable cells hydrolyze calcein-AM to fluorescent calcein via intracellular esterases, whereas propidium iodide penetrates membrane-compromised cells and binds DNA. Dual fluorescence allows discrimination of live and dead populations.
Storage
Shipped at 4°C. Upon delivery store at -20°C.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
