Chicken Intestinal Organoid System (A334707)

Chicken Intestinal Organoid System

This kit allows the culture of chicken intestinal organoids, supporting the study of intestinal biology and tissue organization.

Cell culture, 3D Cell Culture, Organoids

The organoid culture assay is based on the ability of primary tissue-derived cells to self-organize into three-dimensional structures when provided with an appropriate extracellular matrix and a defined, lineage-specific growth environment. Fresh tissue is first processed using a controlled enzymatic digestion solution that releases viable stem and progenitor cell clusters while preserving surface receptors and cellular polarity. The resulting fragments are embedded in a matrix such as Matrigel, which functions as a scaffold that supports 3D architecture and enables signaling through integrins and basement-membrane interactions. The cultures are then supplied with a specialized organoid medium containing niche-mimicking growth factors, small molecules, and nutrients that maintain stemness while permitting tissue-specific differentiation. During routine expansion, a gentle passaging enzyme is used to fragment mature organoids into smaller units without compromising viability, allowing serial propagation over many passages. For biobanking, a cryopreservation formulation containing permeable and non-permeable cryoprotectants is applied to prevent ice-induced damage and maintain long-term genomic and functional stability. After thawing, a recovery buffer restores isotonic balance and supports rapid re-establishment of 3D growth. Together, this workflow enables the continuous generation, expansion, freezing, and re-culturing of physiologically relevant organoids that retain genetic, phenotypic, and microenvironmental features of the source tissue, making the system suitable for disease modeling, drug screening, and personalized research applications.

The kit contains Brain organoid medium A in a 100 mL volume, designed to provide the nutrients and growth factors needed for brain organoid expansion. It can be stored at +4 °C for short-term use for up to three months or frozen at -20 °C for one year. Primary Buffer B2 (250 mL) is included to support sample preparation and early culture maintenance and remains stable for two years when stored at 4-20 °C. Primary tissue digestive juice C (50 mL) is supplied for gentle enzymatic dissociation of brain tissue and is stable at 4-20 °C for one year. The kit also provides Organoid passage digestive juice D (50 mL), used to dissociate mature organoids during subculturing, along with Tissue preservation solution E (100 mL) for temporary storage of samples prior to processing. Organoid Cryopreservation Solution F (20 mL) is included for long-term freezing of organoids, and Passage Buffer G2 (250 mL) is provided for washing and resuspension during organoid transfers, both stable at 4-20 °C for up to two years.

Liquid

Shipped at 4°C. Store at 4°C with a shelf life of 3 months; store at -20°C with shelf life of 1 year.

Proper storage is required to maintain reagent performance. Chicken small intestine organoid medium A should be kept at 4 °C and used within 1 month, or stored at -20 °C for long-term use, avoiding more than two freeze-thaw cycles. Tissue preservation solution E and digestion solution C must also be stored at -20 °C under the same conditions to maintain cell activity. Refer to the Product Manual for complete instructions.