This matched antibody pair is specifically developed for the sensitive and selective detection of Dengue Virus NS1 glycoprotein. The pair consists of a high-affinity capture antibody and a carefully optimized detection antibody that recognize distinct, non-overlapping epitopes on the NS1 antigen. The antibodies are validated for compatibility in lateral flow assay (LFA) formats, ensuring strong signal generation with minimal background. This product is suitable for rapid diagnostic test development, including point-of-care applications targeting early-stage Dengue infection
Applications
Lateral Flow Assay (LFA)
Principle of Assay
In a lateral flow assay, the labeled detection antibody binds Dengue NS1 antigen in the sample to form a complex. This complex migrates along the membrane by capillary action and is captured by the immobilized capture antibody at the test line, producing a visible signal proportional to antigen concentration.
Sample Type
Serum, plasma, whole blood
Specificity
Dengue NS1 is an early diagnostic marker that can be detected from the first day of fever and typically remains present for up to one week. Dengue-specific IgM antibodies generally become detectable between one and five days following the onset of infection. Importantly, NS1 antigen is detectable in both primary and secondary infections and across all four dengue virus serotypes.
Conjugate
Unconjugated
Purity
>95%.
Concentration
1.2mg/ml + 3.4mg/ml
Product Form
Supplied in liquid format. Please note that the size ordered refers to the total combined quantity of both antibodies.
Formulation
Clone 24/c: Supplied in Phosphate Buffered Saline with Sodium Nitrate. Clone 2/b: Supplied in Phosphate Buffered Saline with Sodium Nitrate.
Storage
Shipped and stored at 4°C.
General Notes
This paired monoclonal antibody set has been developed for use in Dengue NS1 antigen lateral flow immunoassays. For optimal performance, it is recommended to conjugate clone 24/c at a gold concentration of 10 µg/mL (OD 1.2–1.5), and to apply clone 2/b as the capture antibody at a coating concentration of 1.2 µg/cm. Assay sensitivity is directly proportional to the optical density of the conjugated gold. A sample volume of 80–90 µL is recommended
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Schematic representation of a lateral flow assay using a matched antibody pair. Following application to the sample pad, the sample rehydrates labeled detection antibodies, which bind the target analyte if present. The resulting complexes migrate along the nitrocellulose membrane by capillary action. At the test line, immobilized capture antibodies bind the analyte-detection antibody complex, forming a sandwich and generating a visible signal. Excess labeled detection antibodies continue to migrate and are captured at the control line by anti-species antibodies, confirming proper assay function, while the absorbent pad maintains continuous flow across the strip. The analyte is represented by a blue shape, the detection antibody by a purple Y conjugated to a gold particle, the capture antibody by a blue Y, the control line antibody by a green Y, and the label by a gold particle (e.g. colloidal gold or latex).
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