This matched antibody pair is specifically developed for the sensitive and selective detection of Dengue Virus NS1 glycoprotein. The pair consists of a high-affinity capture antibody and a carefully optimized detection antibody that recognize distinct, non-overlapping epitopes on the NS1 antigen. The antibodies are validated for compatibility in lateral flow assay (LFA) formats, ensuring strong signal generation with minimal background. This product is suitable for rapid diagnostic test development, including point-of-care applications targeting early-stage Dengue infection
Applications
Lateral Flow Assay (LFA)
Principle of Assay
In a lateral flow assay, the labeled detection antibody binds Dengue NS1 antigen in the sample to form a complex. This complex migrates along the membrane by capillary action and is captured by the immobilized capture antibody at the test line, producing a visible signal proportional to antigen concentration.
Sample Type
Serum, plasma, whole blood
Conjugate
Unconjugated
Purity
95% as determined by SDS-PAGE (coomassie staining).
Concentration
3.6mg/ml
Product Form
Supplied in liquid format. Please note that the size ordered refers to the total combined quantity of both antibodies.
Formulation
Dengue NS1 Gold Conjugation Antibody: Supplied in Phosphate Buffered Saline with Sodium Nitrate, Dengue NS1 Membrane Coating Antibody: Supplied in Phosphate Buffered Saline with Sodium Nitrate.
Storage
Shipped at 4°C. Short term store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
General Notes
Assay conditions involve the use of freshly prepared gold nanoparticles (within 1–2 days) exhibiting high particle size uniformity (?max 525–529 nm, OD 1.2–1.4), followed by antibody conjugation at pH 8.5 for 6–8 hours, and subsequent blocking in which the anti-dengue NS1-conjugated colloidal gold is resuspended to an optical density of 8.0–8.2 and stabilized with a final casein concentration of 0.3–0.4%.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Schematic representation of a lateral flow assay using a matched antibody pair. Following application to the sample pad, the sample rehydrates labeled detection antibodies, which bind the target analyte if present. The resulting complexes migrate along the nitrocellulose membrane by capillary action. At the test line, immobilized capture antibodies bind the analyte-detection antibody complex, forming a sandwich and generating a visible signal. Excess labeled detection antibodies continue to migrate and are captured at the control line by anti-species antibodies, confirming proper assay function, while the absorbent pad maintains continuous flow across the strip. The analyte is represented by a blue shape, the detection antibody by a purple Y conjugated to a gold particle, the capture antibody by a blue Y, the control line antibody by a green Y, and the label by a gold particle (e.g. colloidal gold or latex).
Publishing research using Anti-Dengue Virus NS1 Glycoprotein Matched Antibody Pair (A58136)? Please let us know so that we can list the citation on this page.
